I think you may be surprised if someone was to do a relative and repeatable fuel economy test with 2 identical trucks with the exception that one has a 3.21 rear end vs the other with a 3.92 rear end. At 80MPH, your truck is fighting a huge amount of aerodynamic drag. It takes the same amount of energy to move your truck through the air at those speeds, regardless of what rear end is pushing it. Energy is created by fuel. With the 3.21, you'd be turning 1800 RPM vs 2200 RPM with the 3.92. The difference in friction from the rotating assembly between those 2 RPMs would be negligible. The only other significant contributing factor would be engine efficiency vs RPM vs throttle position. Is the 5.7 Hemi engine significantly more efficient at 1800 RPM than 2200? I'd say no. It may be a common misconception, but lower RPM does not always mean better fuel economy, and in this case, there may not be a substantial difference in fuel economy at 80MPH at 2200RPM vs 1800. My guess would be less than 0.5 MPG difference.
Thinking through this some more, there is an easy way to test this theory on a truck with a 3.21 rear end. There is a 25% difference in the ratio of 8th gear vs 7th gear. 7th gear on a truck with a 3.21 rear is very similar to a truck with a 3.92 rear in 8th gear. While cruising at 80MPH, monitor your MPG on a flat stretch of road, then shift to 7th while maintaining 80 and observe the difference. I'd be interested in knowing what the difference is. I'm sure some others would as well. Anyone out there with a 3.21 rear willing to do the test? It might help some of us sleep better at night knowing that we're not missing out on much of a FE improvement........or not depending on the results.
I may try it out, but it will be like going from a 3.92 to a 4.90